Ecology and the rapd techniques used to assess the genetic diverstiy in peterolobium hexapetalum, A scrambling medicinal shrub in maruthamalai and chennimalai hills, the western ghats
By: Sharmila, S
.
Contributor(s): Akilandeswari, D | Ramya, E. K.
Publisher: M P Innovare Academic Sciences Pvt Ltd 2019Edition: Vol. 2(3).Description: 72-76p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
International journal of pharmacy and pharmaceutical scienceSummary: Objective:
To investigate the ecological and
genetic diversity, climatic factors,
edaphic factors morphological and reproductive characters
and
RAPD analysis of medicinal plant species
Pterolobium hexapetalum
in two hills viz., Maruthamalai (arid) and Chennimalai (very arid), which is
located in Coim
batore and Erode districts, Tamil Nadu.
Method
s:
The present research was carried out by using
a random amplified polymorphic DNA (RAPD) analysis was made to determine the genetic
variation between the two populations of the medicinal shrub,
Pterolobium he
xapetalum
in an environmental gradient. Among the five primers
tested, the OPN7 (80 %) and OPN17 (71.4 %) produced higher polymorphism was used in RAPD analysis.
Result
s:
The results
of RAPD analysis showed the presence of 51 individual bands were formed,
out of which, 29 were polymorphic bands which
showed the existence of genetic variation between populations. A dendrogram was constructed based on Jaccard’s coefficient to
determine the
degree of genetic relationship among the two populations and analysed
. The primers OPN7 and OPN17 were clustered together at a genetic distance
level 10
. Considering the elevation and proximity, the temperature ranges from 18
°C to 37.6
°C in Maruthamalai hill and 20
°C to 39.4
°C in
Chennimalai hill.
Conclusio
n:
From the morphoecological studies the results indicated that both arid and very arid climatic conditions showed slight differences
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2018563 |
Objective:
To investigate the ecological and
genetic diversity, climatic factors,
edaphic factors morphological and reproductive characters
and
RAPD analysis of medicinal plant species
Pterolobium hexapetalum
in two hills viz., Maruthamalai (arid) and Chennimalai (very arid), which is
located in Coim
batore and Erode districts, Tamil Nadu.
Method
s:
The present research was carried out by using
a random amplified polymorphic DNA (RAPD) analysis was made to determine the genetic
variation between the two populations of the medicinal shrub,
Pterolobium he
xapetalum
in an environmental gradient. Among the five primers
tested, the OPN7 (80 %) and OPN17 (71.4 %) produced higher polymorphism was used in RAPD analysis.
Result
s:
The results
of RAPD analysis showed the presence of 51 individual bands were formed,
out of which, 29 were polymorphic bands which
showed the existence of genetic variation between populations. A dendrogram was constructed based on Jaccard’s coefficient to
determine the
degree of genetic relationship among the two populations and analysed
. The primers OPN7 and OPN17 were clustered together at a genetic distance
level 10
. Considering the elevation and proximity, the temperature ranges from 18
°C to 37.6
°C in Maruthamalai hill and 20
°C to 39.4
°C in
Chennimalai hill.
Conclusio
n:
From the morphoecological studies the results indicated that both arid and very arid climatic conditions showed slight differences
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